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Training event 6: Mass spectrometry & Standards for quality management systems

Training event 5: Mass spectrometry and microarrays for glycan analysis & Standards for quality management systems
© INRA

The sixth WallTraC training event was held in Nantes, France from 21 to 23 May 2014. The event was hosted by the project coordinator INRA Angers Nantes research centre. Training providers included Dr. Marie-Christine Ralet, Dr. Hélène Rogniaux, David Ropartz, and Laure Lanet.

Training programme

Scientific training: This scientific training aimed at giving the opportunity to learn how plant cell wall-derived oligosaccharidic substrates can be generated and purified and how they can be characterized by mass spectrometry. This training included courses on principles of mass spectrometry and on mass spectrometry for oligosaccharides structure analysis. Practical sessions gave ESRs/ERs direct hands-on experience of oligosaccharides purification and mass spectrometry.

Complementary skills training: INRA dedicated human resources presented "Standards for quality management systems" issues. The course gave an overview of ISO 9001, which is now the most widely recognized standard for Quality Management. Courses provided an introduction to the broad topic area of quality standards and certification including: how to define quality, the development of quality system standards, structure of the ISO 9001:2000 series, and assessment & certification procedures. Finally, it gave an introduction to occupational health and safety in research.

 Presentation title

 Lecturer

Day 1

 System of quality management, organizational tool
for improving science and research

 D.Ropartz

 The growing role of occupational safety and health in research

 L.Lanet

 PCW polysaccharides: extraction, purification,
and partial degradation prior to MS analysis

 MC.Ralet

 Introduction to practicals

 MC.Ralet

Day 2

 Maldi imaging

 H.Rogniaux

 Practical 1

 D.Ropartz

 Coupling

 D.Ropartz

 Day 3

 MS/MS

 H.Rogniaux

 Practical 2

 D.Ropartz

They talk about the event

WallTraC sixth training event was hosted by INRA in France. It provided the basis to analyse oligosaccharides by mass spectrometry, as well as an introduction to quality management and to occupational health & safety. Mercedes, and Praveen, PhD student at U.Leeds and Max Planck I., respectively gave us their impression at the end of the event:

“We have just had a superb training at INRA. Our non-scientific training was run by David Ropartz and Laura Lanet who gave us an overview on quality management and health and safety.

We learnt what quality management is and how quality standards, such as ISO 9001, are developed and put into practice. Also, we were shown how important health and safety is in a workplace, we were presented with real examples which made very easy to understand how to identify hazards in our work environment, take precautions and, thus, prevent accidents.

I think non—scientific training like these lectures is important for our training, and these talks in particular were very clear and provided us with very valuable knowledge for us as PhD students.” Mercedes.

Mercedes at TE6
Praveen at TE6

"The training event was well organised and started off with a good introductory lecture on the various kinds of Mass spectrometry tools used at INRA and how they can be useful in various fields of plant research especially in plant cell walls.

This was followed by a brief and simple tutorial on how to perform structural linkage analysis on pectin’s and hemicelluloses which was of more interest to me. In the end the training event proved to be quiet useful as I had the chance to learn and apply a few tips and tricks of Mass spectrometry into the plant cell wall field.

Moreover, INRA holds a fantastic working atmosphere and some experts in the field of mass spectrometry which made it an ideal choice to organise this training event." Praveen