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Training event 3: Plant cell walls molecular probes & Ethics

Training event 3: Plant cell walls molecular probes & Ethics
© University of Leeds

The third Training Event was hosted by the University of Leeds in Leeds, United Kingdom from 14 to 16 January 2013. Training providers included Prof. Paul Knox (U.Leeds), Dr. Alice Temple (U.Leeds), Sue Marcus (U.Leeds), Prof. William Willats (U.Copenhagen) and Olivier Tranquet (INRA).

Training programme

The training included both scientific and non-scientific aspects. The training programme is detailed below:

Scientific training: The training included theoretical seminar sessions providing the basis for the usage of molecular probes (topic of WP3). In practical sessions, ESRs/ERs gained direct hands-on experience of cell wall immunocytochemistry and microscopy. Training covered strategies for antibody production, use of antibodies and CBMs in immunolocalization procedures. It also covered the theoretical and practical aspects of cell wall imaging and the capture, preparation and the presentation of micrographs in scientific publications.

Complementary skills training: Dr. Alice Temple, Research Ethics Training and Development Officer at University of Leeds presented "Ethics" issues for researchers. The course covered general ethics sessions including data mismanagement, scientific integrity, conflicts of interest (academia/industry), public engagement with science and more specific elements such as use of animals technology.

 Session title


14 January 2013 - Ethics for researchers

 Introduction to Ethics component: scientific integrity, conflicts of interest and authorship

 Alice Temple, U.Leeds

 Management of research data

 Alice Temple, U.Leeds

 Research with animals

 Alice Temple, U.Leeds

 Public engagement of science. Societal and Ethical impacts

 Alice Temple, U.Leeds

15 January 2013 - Molecular Probes for Plant Cell Walls

 Molecular probes for the analysis of cell wall polysaccharides – Antibodies

 Paul Knox, U.Leeds

 Probes practical session I: Set up immunolabeling of sections

 Sue Marcus, U.Leeds

 Molecular probes for the analysis of cell wall polysaccharides - CBMs

 Paul Knox, U.Leeds

 Probes practical session II

 Sue Marcus, U.Leeds

 Using cell wall probes in immunocytochemistry & microscopy

 Paul Knox, U.Leeds

 Practical session III. Examination of labelled samples with microscope

 Sue Marcus, U.Leeds

16 January 2013 - Molecular Probes for Plant Cell Walls

 Preparation of microscope images presentation

 Paul Knox, U.Leeds

 Probes and glycan microarrays

 William Willats, U.Copenhagen

 Round table discussions:
• Ethics
• Rat vs Mouse vs CBMs.
• Cross reactions and specificity
• General strategies for cell wall imaging

 Paul Knox, U.Leeds; William Willats, U. Copenhagen; Marie-Christine Ralet and Olivier Tranquet, INRA

They talk about the event

WallTraC third training event was hosted by the University of Leeds in England. It provided the basis for the usage of molecular probes and covered different topics related to ethics in science such as the use of animals and scientific integrity. Dhivyaa Rajasundaram, PhD student at the University of Potsdam, and Kate Cameron, PhD student at the University of Lisbon, gave us their impression at the end of the event:

"The scientific training event was well-balanced with the lectures providing theoretical background on the various aspects of antibody production, cell wall immunocytochemistry and microscopy.
Instead of merely sitting through lectures, emphasis was laid on practical aspects of the lectures covered to reinforce the scientific concepts. The hands on experience on immunolabelling and use of confocal microscopy to generate images interested me the most. Professor Paul gave a clear account of the presentation of micrographs in research papers and how to avoid the temptation of image manipulations.
I found the overall experience very informative as it gave me an insight into what it takes to work in the lab to generate data." Dhivyaa

Dhivyaa Rajasundaram at Training Event 3


Kate Cameron at Training Event 3

“I really enjoyed the complimentary skills training as I have always found bioethics very interesting.
The training was interactive and involved a lot of open discussions which is always a nice alternative to conventional training. It was a great opportunity to enhance communication between all members of our WallTraC group and to learn the ideas of others for addressing ethical concerns in our different areas of research.
I have no doubt I will come across some of the situations discussed and it is nice to have an idea on how best to approach them ” Kate.